Presence and distribution of double-stranded RNA elements in the white root rot fungus Rosellinia necatrix |
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Authors: | M Arakawa H Nakamura Y Uetake N Matsumoto |
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Institution: | (1) Bio-oriented Technology Research Advancement Institution, National Institute for Agro-Environmental Sciences, Tsukuba, Japan, JP;(2) National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non dai, Tsukuba 305-8604, Japan Tel. +81-298-38-8267; Fax +81-298-38-8267 e-mail: nowmat@niaes.affrc.go.jp, JP |
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Abstract: | Double-stranded (ds)RNA of various types was detected in 65 (21.8%) of 298 isolates from vegetative hyphae of Rosellinia necatrix by electrophoresis, but dsRNA was not detected from 39 ascosporic isolates. There were 45 distinct dsRNA profiles in the
65 isolates: they varied in the number of electrophoretic bands from 1 to 12 and in size from less than 1000 bp to more than
10 kbp. Each dsRNA profile was unique to each locality. dsRNAs having the same profiles were restricted to isolates of the
same mycelial compatibility groups (MCG) from the same trees, with an exception where different profiles were detected in
different isolates of the same MCGs.
Received: May 7, 2001 / Accepted: September 5, 2001 |
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Keywords: | double-stranded RNA (dsRNA) Mycelial compatibility group (MCG) Rosellinia necatrix White root rot |
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