Cross-linking of horseradish peroxidase adsorbed on polycationic films: utilization for direct dye degradation |
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Authors: | Gulay Bayramoglu Begum Altintas M Yakup Arica |
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Institution: | Biochemical Processing and Biomaterial Research Laboratory, Faculty of Sciences, Gazi University, Teknik Okullar, 06500, Ankara, Turkey, g_bayramoglu@hotmail.com. |
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Abstract: | Horseradish peroxidase (HRP) was immobilized on the polyaniline (PANI) grafted polyacrylonitrile (PAN) films. The maximum HRP immobilization capacity of the PAN-g-PANI-3 film was 221?μg/cm(2). The HRP-immobilized PAN-g-PANI-3 film retained 79?% of the activity of the same quantity free enzyme. The HRP-immobilized PAN-g-PANI-3 film was operated for the decolorization of two different benzidine-based dyes in the presence of hydrogen peroxide. The maximum decolorization grade was obtained at pH 6.0 for both dyes. The HRP-immobilized PAN-g-PANI-3 film was very effective for removal of Direct Blue-53 compared to Direct Black-38 from aqueous solutions. The immobilized HRP exhibited high resistance to proteolysis by trypsin compared to the free counterpart. Immobilized HRP preserved 83?% of its original activity even after 8?weeks of storage at 4?°C, while the free enzyme lost its initial activity after 3?weeks of storage period. |
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