Tuning multi/pluri-potent stem cell fate by electrospun poly(L-lactic acid)-calcium-deficient hydroxyapatite nanocomposite mats |
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Authors: | D'Angelo Francesco Armentano Ilaria Cacciotti Ilaria Tiribuzi Roberto Quattrocelli Mattia Del Gaudio Costantino Fortunati Elena Saino Enrica Caraffa Auro Cerulli Giuliano Giorgio Visai Livia Kenny Josè Maria Sampaolesi Maurilio Bianco Alessandra Martino Sabata Orlacchio Aldo |
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Institution: | Department of Experimental Medicine and Biochemical Sciences, Section of Biochemistry and Molecular Biology, University of Perugia, Via del Giochetto, Perugia, Italy. |
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Abstract: | In this study, we investigated whether multipotent (human-bone-marrow-derived mesenchymal stem cells hBM-MSCs]) and pluripotent stem cells (murine-induced pluripotent stem cells iPSCs] and murine embryonic stem cells ESCs]) respond to nanocomposite fibrous mats of poly(L-lactic acid) (PLLA) loaded with 1 or 8 wt % of calcium-deficient nanohydroxyapatite (d-HAp). Remarkably, the dispersion of different amounts of d-HAp to PLLA produced a set of materials (PLLA/d-HAp) with similar architectures and tunable mechanical properties. After 3 weeks of culture in the absence of soluble osteogenic factors, we observed the expression of osteogenic markers, including the deposition of bone matrix proteins, in multi/pluripotent cells only grown on PLLA/d-HAp nanocomposites, whereas the osteogenic differentiation was absent on stem-cell-neat PLLA cultures. Interestingly, this phenomenon was confined only in hBM-MSCs, murine iPSCs, and ESCs grown on direct contact with the PLLA/d-HAp mats. Altogether, these results indicate that the osteogenic differentiation effect of these electrospun PLLA/d-HAp nanocomposites was independent of the stem cell type and highlight the direct interaction of stem cell-polymeric nanocomposite and the mechanical properties acquired by the PLLA/d-HAp nanocomposites as key steps for the differentiation process. |
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