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Characterization of microsatellite loci in two closely related Lissotriton newt species
Authors:Aurélie Johanet  Damien Picard  Trenton W J Garner  Deborah A Dawson  Ramiro Morales-Hojas  Robert Jehle  Didier Peltier  Christophe Lemaire
Institution:1. Laboratoire Paysages et Biodiversité, UFR Sciences, Université d’Angers, 2 bd Lavoisier, Angers, France
8. UMR PaVé, INRA, 42 rue Georges Morel, Beaucouzé, France
2. Institute of Zoology, Zoological Society of London, Regent’s Park, London, NW1 4RY, UK
3. Department of Biology, University of Leicester, Leicester, LE1 7RH, UK
4. Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK
5. Laboratório de Evolu??o Molecular, Instituto de Biologia Molecular e Celular, Porto, Portugal
6. School of Environment and Life Sciences, University of Salford, Greater Manchester, UK
7. UMR GenHort, UFR Sciences, Université d’Angers, 2 bd Lavoisier, Angers, France
Abstract:We have developed eight di- and tetranucleotide Lissotriton microsatellite markers. Eight loci were polymorphic in the palmate newt Lissotriton helveticus and six were polymorphic in the smooth newt L. vulgaris. Polymorphism detected in 33 and 37 individuals per species ranged from 3 to 15 alleles. These markers are suitable for the investigation of population structure, genetic variation and taxonomic identification in the two focal species, and may also be of use in other Lissotriton–Triturus species.
Keywords:
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