Characterization of YS-27, an axenic Korean strain of Entamoeba histolytica |
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| Authors: | Park S J Yong T S Chang J K Im K Soh C T |
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| Institution: | Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea. sjpark615@yumc.yonsei.ac.kr |
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| Abstract: | Characterization of YS-27, an axenic Entamoeba strain, was performed by three different laboratory methods. Zymodeme analysis using starch gel electrophoresis and PCR with species-specific primers showed that YS-27 is a pathogenic Entamoeba which belongs to the group II zymodeme. Pathogenicity of YS-27 was further confirmed by observing the formation of liver abscess in Mongolian gerbils. These results showed that YS-27 is E. hisolytica. |
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| Keywords: | Entamoeba histolytica zymodeme PCR liver abscess |
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