A simple and sensitive enzyme-mediated assay of biotin. |
| |
| Authors: | Z Huang R P Haugland D Szalecka R P Haugland |
| |
| Institution: | Molecular Probes, Inc., Eugene, OR 97402. |
| |
| Abstract: | Free biotin was quantitated by a competition by coating biotin-bovine serum albumin conjugate on a polystyrene microplate for binding to avidin-beta-galactosidase conjugate. The enzyme conjugate remaining on the plate surface as a result of the competition was detected by reaction with one of the following fluorogenic substrates, resorufin beta-D-galactoside and fluorescein di-beta-D-galactoside, in a fluorescence plate reader. Free biotin as little as 0.1 nmol can be routinely detected. |
| |
| Keywords: | |
|
|
