人参根提取物增强巨噬细胞RAW264.7的自噬水平并提高其增殖和吞噬能力

研究人参根提取物对巨噬细胞RAW264.7的增殖能力、吞噬能力和自噬水平的影响以及其相关性.用细胞计数试剂(CCK-8)检测不同浓度的人参根以及加入对巨噬细胞RAW264.7增殖的影响;采用中性红吞噬实验检测人参根提取物对巨噬细胞吞噬活性的影响;采用吖啶橙染色法(AO染色法)检测自噬体的形成;采用免疫印迹法(Weste...

Ginseng root extract enhanced the autophagy level of macrophage RAW264.7 and increased its proliferation and phagocytosis

The aim of this study is to investigate the effect of ginseng root extract on RAW264.7 cells proliferation,phagocytosis and autophagy level and their correlation.Cell counting reagent(CCK-8)was used to detect the effect of ginseng root extract in cell proliferation of RAW264.7 cells.Neutral red phagocytosis experiment was used to detect the effect of ginseng root extract in phagocytic activity of RAW264.7 cells.The formation of autophagosome was detected by acridine orange staining(AO staining).Western blot was used to detect the expression changes of autophagy related proteins LC3B and ATG7 and the phosphorylation changes of autophagy related proteins AMPK,AKT,mTOR and ULK1.Autophagy regulator rapamycin and CQ were introduced to research the relationship of autophagy,cell proliferation and phagocytosis induced by ginseng root extract.The Results shown that different concentration of ginseng root extract could promote the proliferation and phagocytosis of RAW264.7 cells,compared with blank control group.Ginseng root extract could increase the number of acidic autophagosomes in Raw264.7 cells.The expressions of LC3B,ATG7 and the protein phosphorylation levels of AMPK and ULK1 were increased in ginseng root extract treated cells.Meanwhile,the phosphorylation levels of AKT and mTOR were decreased.Rapamycin further enhanced the phagocytosis of macrophages,while CQ attenuated the improvement of macrophage phagocytosis induced by ginseng root extract.In conclusion,ginseng root extract may enhance the autophagy level of macrophagy RAW264.7,increase cell proliferation and phagocytosis activity,and has the potential effect of activating macrophages to enhance the immunity of the body.