草甘膦对茶树叶片主要生化成分的影响

以金观音茶树为试材,将草甘膦定量施于栽培基质,通过观察叶片的表观药害,并采用超高效液相串联质谱方法测定草甘膦施用前(0天)和施用后(7天和33天)叶片中的农药残留(草甘膦及氨甲基膦酸)及生化成分(游离氨基酸、儿茶素和生物碱)的含量变化,从而科学地评估草甘膦的使用对茶叶质量安全的影响.结果表明:草甘膦施用33天,茶树叶片...

Effect of glyphosate on main biochemical components of tea leaves

Taking Camellia sinensis‘Jin-guanyin’as test material,glyphosate was quantitatively applyed on tea plants cultivation substrate.By observing the apparent phytotoxicity of tea leaves,and using ultra-high performance liquid phase tandem mass spectrometry to determine the pesticide residues(glyphosate and aminomethylphosphonic acid)and main quality components(free amino acids,catechins and alkaloids)in the tea leaves before(0 d)and after(7 d and 33 d)glyphosate applying,to explore the impact of glyphosate application on the quality and safety of tea production.The results showed that:glyphosate was applied for 33 days,and there was no obvious sign of phytotoxicity in tea leaves,but glyphosate and its metabolite aminomethylphosphonic acid were still detected;The total amount of free amino acids and catechins decreased after the application of glyphosate,while the total amount of alkaloids first increased and then decreased;Multivariate statistical analysis showes,there are obvious differences of theanine,caffeine,epigallocatechin gallate,glutamine,epicatechin gallate,epicatechin,arginine,aspartic acid,glutamic acid and epigallocatechin between the sample groups before and after glyphosate application.In summary,the application of glyphosate in tea gardens is not easy to cause phytotoxicity in the tea leaves,but it can remain in the leaves of tea plants for a long time and significantly change the content of free amino acids,catechins and alkaloids in tea leaves.