姜黄在理血方剂中的配伍规律及系统药理机制分析

姜黄可以有效治疗各种疾病,本研究使用广义规则归纳(GRI)算法分析了《中药方剂大词典》中含姜黄的理血方剂配伍规律.通过利用生物信息学数据挖掘技术和MTT、Elisa和Western blotting实验验证了姜黄关键配方的系统药理机制.发现"姜黄-川芎-当归"配方可以代表姜黄在理血方剂中的关键组方.PPI网络和KEGG...

Analysis of the compatibility laws of Jianghuang in blood-regulating prescriptions and systemic pharmacological mechanism

Jianghuang(JH)could be effective for the treatment of various diseases.The compatibility laws of blood-regulating formulae containing JH from the Chinese Traditional Medicine Formula Dictionary were analyzed using a generalized rule induction(GRI)algorithm implemented.System pharmacological mechanisms are traced by leveraging data mining of bioinformatics databases and MTT cell proliferation assay,Elisa and Western blotting analysis.We found that the JH-CX-DG formula(Jianghuang-Chuanxiong-Danggui)could represent a key formula containing JH in blood-regulating TCM formulae.The PPI network and KEGG pathway enrichment analysis showed that the JH-CX-DG formula possess potential pharmacological effects including anti-inflammatory,improving microcirculation,and anti-tumor through the regulation of multiple pathways including PI3K/Akt,MAPK,Toll-like receptor,T cell receptor,EGFR,VEGFR,Apoptosis,HIF-1(P<0.05).The MTT assay,Elisa and Western blotting showed that the“JH-CX-DG”formula can inhibit human lung cancer A549 cells proliferation and down-regulate the expression of p-EGFR,p-PI3K,p-Akt,VEGF and HIF-1α.The JH-CX-DG formula can exert beneficial pharmacological effects through multi-target and multi-pathway interactions.It maybe be effectively administered for the treatment of inflammatory diseases,microcirculation disorders,cardiovascular disease,and cancer.We found a new effective drug formula through analyzing the compatibility law and systemic pharmacological mechanism of JH.Our study provides a theoretical basis and directions for subsequent research on the JH-CX-DG formula.