桂皮醛对DSS诱导的溃疡性结肠炎小鼠的保护作用研究

为探讨桂皮醛(CA)对实验性溃疡性结肠炎(UC)小鼠的保护作用及其初步机制,用柳氮磺胺吡啶(300 mg/kg)做为阳性对照,CA分别以150、250、500 mg/kg的剂量对3％葡聚硫酸钠(DSS)诱导的UC小鼠进行灌胃治疗.观察小鼠给药前后状态的变化并进行DAI评分,HE染色法观察小鼠结肠组织形态的变化并进行病理...

Protective effects of cinnamaldehyde on DSS induced UC mice

To explore the protective effects and its mechanism of cinnamaldehyde(CA)on ulcerative colitis(UC)mice.Sulfasalazine(300 mg/kg)was used as the positive control,and CA was administered to the UC mice which was induced by 3%dextran sodium sulfate(DSS)at 150,250,500 mg/kg doses.Observe the changes in the state of the mice before and after the administration and perform the DAI score,and the morphological changes of mice colon were observed by HE staining and pathological evaluation was performed;ELISA kit and Western blot technology were used to detect the changes of inflammatory factors in the serum of mice and the expression of inflammation-related proteins in the colonic epithelium of mice.The results showed that CA could protect the colonic tissue morphological integrity and improve colonic mucosal injury in UC mice within the experimental dose range;Increase the levels of IL-4,IL-10,reduce the levels of IL-6,IL-8 and TNF-αin serum;up-regulate the expression of NF-κB in the cytoplasm,and inhibit the level of phosphorylated STAT3.In summary,CA has a protective effect on UC mice,which could reduce the degree of colon injury and inhibit inflammation.The more in-depth mechanism need to be further study.