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Microsatellite markers for <Emphasis Type="Italic">Caesalpinia echinata</Emphasis> Lam. (Brazilwood), a tree that named a country
Authors:Sônia Cristina Oliveira Melo  Fernanda Amato Gaiotto  Fernanda Barbosa Cupertino  Ronan Xavier Corrêa  Alessandra Maria Moreira Reis  Dário Grattapaglia  Rosana Pereira Vianello Brondani
Institution:1.Departamento de Ciências Biológicas,Universidade Estadual de Santa Cruz,Ilheus,Brazil;2.Universidade Católica de Brasília,Brasilia,Brazil;3.Embrapa Recursos Genéticos e Biotecnologia,Brasilia,Brazil;4.Laboratório de Biotecnologia,Embrapa Arroz e Feij?o,Goiania,Brazil
Abstract:Caesalpinia echinata, commonly known as Pau-brasil (Brazilwood), the famous tree that named Brazil is native to the Atlantic forest. Men extensively exploited it ever since discovery and colonial times due to its value as a source of red dye. As a consequence, Brazilwood is a threatened species with populations reduced to small forest fragments. Ten polymorphic microsatellite loci were developed from an enriched genomic library. Using fluorescently-labeled primers, a total of 83 alleles were found after analyzing a sample of 44 trees. These high genetic information content markers should allow detailed investigations of mating systems, gene flow, population structure and paternity in natural populations.
Keywords:SSR  Atlantic forest  Natural population  Allelic diversity  Conservation
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