Immunocytochemical demonstration of GAP-like immunoreactive neuronal elements in the human hypothalamus and pituitary |
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Authors: | J Abe H Okamura Dr Y Ibata A Motoyama I Wakabayashi N Ling W K Paull |
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Institution: | (1) Department of Anatomy, Kyoto Prefectural University of Medicine, Kamikyoku, 602 Kyoto, Japan;(2) Department of Medicine, Nippon Medical School, Bunkyoku, 113 Tokyo, Japan;(3) The Laboratories for Neuroendocrinology, The Salk Institute for Biological Studies, 92037 La Jolla, CA, USA;(4) Department of Anatomy, School of Medicine, University of Missouri-Columbia, 65212, MO, USA |
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Abstract: | Summary GnRH-associated peptide (GAP)-like immunoreactive elements located in the human hypothalamus were investigated by PAP immunocytochemistry
using specific antiserum against pro-GnRH (14–69) OH]. Immunoreactive neuronal perikarya were distributed in the MPOA, PVN
and infundibular nucleus, with the largest numbers of GAP-like immunoreactive perikarya found in the infundibular nucleus.
We also detected the coexistence of GAP-like and GnRH-like immunoreactivities in the same neuronal perikarya in the MPOA by
using a double immunolabelling procedure. In addition to the above regions immunoreactive neuronal perikarya were present
in the region dorsal to the medial mammillary nucleus. GAP-like immunoreactive fibers were distributed in same areas that
immunoreactive perikarya were observed. Many immunoreactive terminals were found adjacent to capillaries in the infundibulum.
Immunoreactive dots, presumably terminals, were observed in the posterior pituitary and these were particularly evident along
the margin adjacent to the anterior pituitary. The distribution pattern and density of GAP-like immunoreactive neuronal elements
are compared with those of other mammalian species. We also compared GAP-like immunoreactive elements with that of GnRH as
has been previously observed in the human hypothalamus. |
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