| 小檗碱激活人结肠癌细胞容积敏感的氯通道 |
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| 作者姓名： | Liu SW Li Y Li HR Ma WB Pan TC Zhu LY Ye WC Wang LW Chen LX |
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| 作者单位： | 暨南大学医学院生理学系;暨南大学医学院药理学系;暨南大学医学院药学院; |
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| 基金项目： | supported by the National Natural Science Foundation of China(No.30771106,30870567,30871267,90913020and U0932004); Natural Science Foundation of Guangdong Province,China(No.7005974) |
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| 摘 要： | 本文旨在研究小檗碱对人结肠癌细胞(SW480)氯通道的作用.采用膜片钳技术记录小檗碱激活的SW480全细胞氯电流,并用高渗和低渗灌流液、以及氯通道阻断剂研究该电流的生理学和药理学特性.结果显示,当细胞处在等渗液中,可在SW480细胞膜上记录到微弱且稳定的背景电流;小檗碱(10 nmol/L)可诱发SW480细胞迅速产生...
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| 关 键 词： | 小檗碱 人结肠癌细胞 氯通道:膜片钳技术 |
Berberine activates volume-sensitive chloride channel in human colorectal carcinoma cells |
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| Liu SW,Li Y,Li HR,Ma WB,Pan TC,Zhu LY,Ye WC,Wang LW,Chen LX.Berberine activates volume-sensitive chloride channel in human colorectal carcinoma cells[J].Acta Physiologica Sinica,2011,63(6):517-524. |
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| Authors: | Liu Shan-Wen Li Yuan Li Hua-Rong Ma Wen-Bo Pan Ting-Cai Zhu Lin-Yan Ye Wen-Cai Wang Li-Wei Chen Li-Xin |
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| Institution: | LIU Shan-Wen 1,LI Yuan 2,LI Hua-Rong 1,MA Wen-Bo 1,PAN Ting-Cai 2,ZHU Lin-Yan 2,YE Wen-Cai 3,WANG LiWei 1,CHEN Li-Xin 2,1 Department of Physiology of Medical College,2 Department of Pharmacology of Medical College,3 College of Pharmacy,Jinan University,Guangzhou 510632,China |
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| Abstract: | The present study aimed to clarify the effect of berberine on the chloride channels in human colorectal carcinoma cells (SW480).The whole-cell patch clamp technique was used to detect the Cl current activated by berberine.The physiological and pharmacological characteristics of the current were clarified by changing the osmotic pressure of extracellular perfusate and applying chloride channel blockers.The results showed that,under isotonic conditions,the background current of SW480 cells was weak and stable... |
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| Keywords: | berberine human colorectal carcinoma cells chloride channels patch-clamp techniques |
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