Enumeration of single IFN-gamma-producing cells in mice during viral and bacterial infection |
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Authors: | A Gessner D Moskophidis F Lehmann-Grube |
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Affiliation: | Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universit?t Hamburg, Germany. |
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Abstract: | A solid phase immunoenzymatic technique was employed for detecting single IFN-gamma-producing cells (IFN-gamma PC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-gamma PC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-gamma PC, but considerably more than 50% were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine. |
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