Protein synthesis in lactating guinea-pig mammary tissue perfused in vitro. II. Biogenesis of milk-fat-globule membrane proteins |
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| Authors: | I H Mather G Bruder E D Jarasch H W Heid V G Johnson |
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| Affiliation: | 1. Division of Membrane Biology and Biochemistry, Institute of Cell and Tumor Biology, German Cancer Research Center, D-6900 Heidelberg, FRG;2. Department of Animal Sciences, University of Maryland, College Park, MD 20742, USA |
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| Abstract: | Guinea-pig mammary tissue was perfused in vitro, radiolabelled with [35S]methionine and intracellular protein precursors of the milk-fat-globule membrane (FGM) recovered by immunoabsorption techniques. Labelled xanthine oxidase was solely detected in post-microsomal supernatants and butyrophilin in carbonate-washed membranes. A major glycoprotein (Gp 55), was initially present in a membrane-bound form, but after longer perfusion times a fraction of this protein was recovered in the post-microsomal supernatant. These results are discussed with reference to formation of the apically-derived FGM. |
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| Keywords: | To whom offprint requests should be sent. Address: Department of Animal Sciences University of Maryland College Park MD 20742 USA. |
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