Differences in compounds released by embryogenic and non-embryogenic suspension cultures of Euphorbia pulcherrima |
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Authors: | K Brandau W Preil R Lieberei |
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Institution: | (1) Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding, Bornkampsweg 31, D-22926 Ahrensburg, Germany;(2) Institute of Applied Botany, University of Hamburg, Marseiller Str. 7, D-20355, Hamburg, Germany |
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Abstract: | Media from embryogenic and non-embryogenic cell suspension cultures were analysed for protein content, electrophoretic protein
patterns, glycoproteins and activity of peroxidases and β-glucosidases in order to characterize the physiological status of
the cultures. On a dry mass basis the amount of extracellular proteins per cell was greater in embryogenic suspensions than
in non-embryogenic suspensions. Non-embryogenic suspensions contained unidentified slimy compounds which were not present
inembryogenic cultures. The extracellular Concanavalin A-specific glycoproteins gave different isoelectric focussing patterns
and thus enabled embryogenic and non-embryogenic cultures to be differentiated. The extracellular peroxidase activity per
cell dry mass was far greater in embryogenic than in non-embryogenic cultures. The isoenzymes differed in number and composition
of the anionic bands. β-glucosidases were found in the same range of activity in both culture types, but the time course of
enzyme activity during cultivation was significantly different. In the embryogenic culture the activity was correlated with
dry mass increase, whereas in the non-embryogenic suspension the activity reached maximum during the linear growth phase.
Polyphenoloxidase which was recently recognized as an intracellular marker for embryogenic stages was not released into culture
media.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | β -glucosidases glycoproteins peroxidases somatic embryogenesis |
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