Paenibacillus strain MP-1: a new source of mutanase |
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Authors: | M. Pleszczyńska M. Marek-Kozaczuk A. Wiater J. Szczodrak |
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Affiliation: | (1) Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland;(2) Department of General Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland |
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Abstract: | A mutan-degrading bacterium, closely related to Paenibacillus curdlanolyticus, was isolated from soil. It produced 0.4 U mutanase ml−1 in 2 days in shake-flask cultures when bacterial mutan was the sole carbon source. Mutanase activity was optimal at pH 6.2 and 45°C over 1 h and was stable between pH 5.8 and 12 at 4°C for 24 h and up to 40°C for 1 h. Mutan produced by Streptococcus mutans was rapidly hydrolyzed by this enzyme. The hydrolysis of mutan (1 g l−1) resulted in 17% saccharification over 2 h and, at the same time, glucan was entirely solubilized. |
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Keywords: | Isolation Mutanase Mutan hydrolysis Paenibacillus |
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