Comparison of RNA- and DNA-based bacterial communities in a lab-scale methane-degrading biocover |
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Authors: | Tae Gwan Kim Kyung-Eun Moon Jeonghee Yun Kyung-Suk Cho |
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Institution: | 1. Department of Environmental Science and Engineering, Ewha Womans University, Seoul, 120-750, Republic of Korea
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Abstract: | Methanotrophs must become established and active in a landfill biocover for successful methane oxidation. A lab-scale biocover with a soil mixture was operated for removal of methane and nonmethane volatile organic compounds, such as dimethyl sulfide (DMS), benzene (B), and toluene (T). The methane elimination capacity was 211?±?40 g?m?2 d?1 at inlet loads of 330–516 g?m?2 d?1. DMS, B, and T were completely removed at the bottom layer (40–50 cm) with inlet loads of 221.6?±?92.2, 99.6?±?19.5, and 23.4?±?4.9 mg m?2 d?1, respectively. The bacterial community was examined based on DNA and RNA using ribosomal tag pyrosequencing. Interestingly, methanotrophs comprised 80 % of the active community (RNA) while 29 % of the counterpart (DNA). Types I and II methanotrophs equally contributed to methane oxidation, and Methylobacter, Methylocaldum, and Methylocystis were dominant in both communities. The DNA vs. RNA comparison suggests that DNA-based analysis alone can lead to a significant underestimation of active members. |
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