A simplified method for the detection of Y chromosome microdeletions in infertile men using a multiplex sequence-tagged site-based amplification |
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| Authors: | Charpenel Christine Guillon Anne-Sophie Dorson Olivier Boucly Catherine Mathieu Bertille Montagnon Martine Merlet Françoise Van Amerongen Geneviève Bisson Jean Pierre de Mazancourt Philippe |
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| Affiliation: | Laboratoire de Biochimie et Biologie Moléculaire, H?pital R Poincaré, 92380 Garches, France. |
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| Abstract: | Sixteen sequence-tagged sites (STSs) were combined in five amplification reactions, to screen for deletions of DNA fragments located within the AZFa, AZFb, and AZFc regions of the Y chromosome. This multiplex strategy is fast and reliable, and most of the azoospermia-associated deletions reported so far are detected with this simplified method. Internal control STSs are included that allow discrimination between deletion and failure of amplification. |
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