Optimization of Phosphatidylinositol-specific Phospholipase C Production Using Response Surface Methodology |
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Authors: | Email author" target="_blank">T?PalvannanEmail author R?Boopathy |
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Institution: | (1) Department of Biotechnology, Kumaraguru College of Technology, 641 006 Coimbatore, Tamil Nadu, India;(2) Department of Biotechnology, Bharathiar University, 641 046 Coimbatore, Tamil Nadu, India |
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Abstract: | Summary Response surface methodology was employed in optimizing the nutrient levels needed towards the optimal production of phosphatidylinositol-specific
phospholipase C enzyme by Bacillus thuringiensis serovar. kurstaki. A 23 factorial central composite experimental design was used. The multiple regression equation, relating the enzyme activity
to the nutrient medium, was used to find the optimum values of glucose, peptone and dipotassium hydrogen phosphate. The optimum
values of these variables for maximal enzyme production were found to be: glucose, 6.5 g l−1; peptone, 5.38 g l−1 and dipotassium hydrogen phosphate, 6.36 g l−1 with the predicted enzyme activity of 0.96 U ml−1. |
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Keywords: | Acetylcholinesterases Bacillus thuringiensis phosphatidylinositol-specific phospholipases C optimization response surface methodology |
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