Cometabolic Degradation of Trichloroethylene by Single- and Two-Phase Systems |
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| Authors: | Tareq Abu Hamed Emine Bayraktar Tanju Mehmetoglu Ülkü Mehmetoglu |
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| Institution: | 1. Arava Institute for Environmental Studies , Hevel Eilot , Israel;2. Dead Sea and Arava Science Center, Tamar Regional Council , Israel;3. Ankara University, Faculty of Engineering, Department of Chemical Engineering , Ankara , Turkey;4. Middle East Technical University, Faculty of Engineering, Department of Petroleum and Natural Gas Engineering , Ankara , Turkey |
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| Abstract: | The cometabolic degradation of trichloroethylene (TCE) by Pseudomonas putida F1 (strain ATCC 700007) at different concentrations was studied in single- and two-phase systems using 2-undecanone as the second organic phase. Toluene vapors were used as the primary growth substrate for Pseudomonas putida F1. The effects of the biomass concentration and the phase ratio on the biodegradation process were investigated. The best biomass concentration and the most suitable phase ratio were found to be 0.462 and 0.025 g/L (vorg/vaq), respectively. In the single-phase system, 36.5 mg/L TCE was degraded completely in 15 hours and only 78% of 55 mg/L TCE was degraded in 27 hours, while in the two-phase system 55 mg/L TCE was degraded completely in 14 hours. The use of the two-phase system not only decreased the biodegradation time of TCE but also prevented the inhibition effect of high concentrations of TCE on the microbial biomass. |
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| Keywords: | Cometabolic biodegradation trichloroethylene Pseudomonas putida F1 two-phase system 2-undecanone |
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