Comparative shoot regeneration responses of diploid brassicas and their synthetic amphidiploid products |
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Authors: | S B Narasimhulu Shyam Prakash V L Chopra |
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Institution: | (1) Biotechnology Centre, Indian Agricultural Research Institute, 110012 New Delhi, India |
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Abstract: | Comparative shoot regeneration responses of three diploid Brassica species, B. campestris (AA), B. nigra (BB) and B. oleracea (CC) and their synthetic amphidiploid combinations have been investigated. The study indicates that A genome has an inhibitory effect on regeneration as evident from significantly low responses of B. juncea (AABB) and B. napus (AACC) combinations, in comparison with regeneration response of B. nigra and B. oleracea. This inhibition may arise from genomic interactions or from the B. campestris cytoplasm interacting negatively with the alien genome. Significant cytoplasmic influence on regenerability has been observed in B. carinata (BBCC) synthesised from reciprocal crosses of B. nigra and B. oleracea.Abbreviations MS
Murashige and Skoog (1962)
- IAA
Indole-3-acetic acid
- NAA
-napthaleneacetic acid
- BA
6-Benzylaminopurine |
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