Effect of endothelin-1 induced ischemia on peroxidative damage and membrane properties in rat striatum synaptosomes |
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| Authors: | P. Viani I. Zini G. Cervato G. Biagini L. F. Agnati B. Cestaro |
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| Affiliation: | (1) Department of Medical Chemistry and Biochemistry, Faculty of Medicine, University of Milano, via Saldini 50, 20133 Milano, Italy;(2) Department of Biomedical Sciences, Section of Physiology, Faculty of Medicine University of Modena, Italy |
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| Abstract: | Synaptosomes obtained from rat striata lesioned by central injection of endothelin-1 (ET-1) were analyzed for the levels of lipid peroxidation products, the susceptibility to lipid peroxidation, the phospholipid and free fatty acid composition and the activity of Na+,K+-ATPase one hour after ET-1 treatment. The intrastriatal injection of ET-1 promoted an increase of endogenous thiobarbituric reactive substances (TBARS), as index of free radical mediated lipid damage, and a greater susceptibility to iron/ascorbate-induced lipid peroxidation. The pattern of free fatty acids showed a significant decrease of arachidonic and docosahexaenoic acid consequent to ET-1 treatment. The analysis of lipid composition showed a significant loss of phospholipids: among phospholipid species, sphingomyelin and phosphatidylethanolamine plasmalogen were particularly reduced by ET-1 treatment. The activity of membrane-bound Na+,K+-ATPase was also significantly reduced in synaptosomes obtained from ET-1 lesioned striata. Taken together these results indicate a significant modification of synaptosomal membrane of ET-1 treated rat striata, possibly due to a free radical mediated damage. |
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| Keywords: | Ischemia lipid peroxidation endothelin-1 |
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