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Influence of impeller speed on citric acid production and selected enzyme activities of the TCA cycle
Authors:T Roukas
Institution:(1) Department of Food Science and Technology, Aristotelian University of Thessaloniki, Box 250, 54006 Thessaloniki, Greece
Abstract:Summary The effect of impeller speed on citric acid production and selected enzyme activities of the TCA cycle was studied. The highest yield of citric acid (28 g/l) was obtained in culture agitated at lower speed (300 rpm). The activity of citrate synthase decreased with the increase of speed of agitation, while the activity of aconitase and isocitrate dehydrogenase increased with the increase in agitation speed.
Keywords:Citric acid  Aspergillus niger  Submerged culture  Stirred tank fermenter
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