Chromosome partition in Escherichia coli requires postreplication protein synthesis. |
| |
Authors: | W D Donachie and K J Begg |
| |
Affiliation: | Department of Molecular Biology, University of Edinburgh, Scotland. |
| |
Abstract: | After inhibition of protein synthesis, the number of nuclear bodies (nucleoids) visible in cells of Escherichia coli B/rA corresponded closely to the number of completely replicated chromosomes. We calculated that nucleoid partition follows almost immediately after replication forks reach the chromosome terminus. We show that such a partition is dependent on protein synthesis and that this may reflect the requirement that cells must achieve a certain minimum length before partition (and subsequent cell division) can take place. |
| |
Keywords: | |
|
|