Bmi-1 在非小细胞肺癌中的表达及相关性探讨

目的:探讨Bmi-1在非小细胞肺癌(NSCLC)组织中的表达及其与临床病理特征的关系。方法:采用RT-PCR检测30例非小细胞肺癌及20例癌旁组织中Bmi-1mRNA的表达情况,同时应用免疫组织化学SP法检测52例非小细胞肺癌及30例癌旁组织中Bmi-1蛋白表达情况。结果:非小细胞肺癌组织中Bmi-1mRNA表达量明显高于癌旁组织(t=5.188,P<0.01)。肺癌组中Bmi-1蛋白的阳性表达率为67.3%(35/52),明显高于癌旁组13.3%(4/30),且表达量差别有高度统计学意义(Z=-4.837,P<0.01);肺癌组中Bmi-1蛋白阳性表达与癌组织的TNM分期及有无淋巴结转移有关(Z=-2.567,-2.366,P<0.05),而与患者的性别、年龄、组织类型、分化程度等无关(P均>0.05);结论:Bmi-1在非小细胞肺癌中的高表达与肿瘤发生发展及转移相关,可成为早期诊断肺癌及判断转移的重要参考指标。

Expression of Bmi-1 in NSCLC and Its Relationship to Clinicopathologic Characters

Objective: To investigate the relationship between the expression of Bmi-1 in nonsmall-eell lung cancer (NSCLC) and elinicopathologic characters. Methods: Reverse transeription-PCR(RT-PCR)was applied to detect Bmi-1 mRNA in 30 of NSCLC speci-men and 20 of tumor-adjacent tissue. Immunohistoehemistry was performed to detect the expression of Bmi-1 in 52 of NSCLC specimen and 30 of tumor-adjacent tissue. Results: The expression of Bmi-lmRNA in NSCLC specimen was remarkably higher than that in tu-mor-adjacent tissue (t=-5.188, P<0.01 ). The positive rote of Bmi-1 expression was 67.3%( 35/52 )in NSCLC specimen and 13.3%(4/30)in tumor-adjacent tissue, respectively. There was a statistically significant difference for the expression of Bmi-lmRNA between NSCLC specimen and tumor-adjacent tissue (Z＝4.837, P<0.01 ). Bmi-1 expression in NSCLC was correlated with both TNM staging and lymph node metastasis ( Z=2.567, -2.366, P<0.05 ), but which had no relationship with sex, age, histotype and differentiation ( P >0.05 ). Conclusion:High expression of Bmi-1 in NSCLC is related with the development and metastasis of tumorigenesis.