IL-4 和IL-12 在宫颈癌中的表达及对紫杉醇过敏的影响

目的:研究IL-4,IL-12在宫颈癌组织中的表达,探讨其对宫颈癌发生及术后对紫杉醇过敏的影响。方法:应用半定量逆反应-聚合酶链反应(RT-PCR)技术检测IL-4mRNA,IL-12p35以及IL-12p40 mRNA在正常宫颈组和宫颈癌组中的表达,并分析两者之间的相关性以对紫杉醇过敏的影响。结果:1.宫颈癌组中IL-4mRNA表达水平高于正常宫颈组,而IL-12p35和IL-12p40mRNA表达低于正常宫颈组,差异有统计学意义(P<0.05);2.在术后给予紫杉醇治疗的宫颈癌患者中,过敏组中IL-4mRNA的表达高于不过敏组;IL-12p35和IL-12p40mRNA则低于后者,差异有统计学意义(P<0.05)。结论:体内IL-12降低和(或)IL-4升高可促进宫颈癌的发生发展增加紫杉醇过敏的发生率。

Significance of IL-4 and IL-12 in Cervical Cancer and Relation toTaxol Allergy

Objective:To investigate the expression of IL-4 and IL-12 in the cervical cancer and to investigate the relationships between the cervical carcinoma and Taxol allergy.Methods:Total RNA was extracted from paraffin-embedded tissues which were from 35 cases ofcervical cancer and 15 cases of normal cervix.The results were analyzed by the quantitation access to densitometric scan of agarose gel electrophoresis photos.Results:1.The expression of IL-4 in the cervical carcinoma was higher than that in normal cervical tissues while the expressions of IL-12p35 and IL-12p40 had the opposite tendency.2.The expression of lL-4mRNA in allergic groups was higher than that in control groups during Taxol therapy with cervical cancer.IL-12p35mRNA and IL-12p40mRNA decreased. Conclusions:The increasing of IL-4 and (or)the dropping of IL-12 might promote cervical diseases and increase the risk of taxol allergy after the operation.