电磁脉冲(EMP)辐照对雄性子代大鼠下丘脑GABAA 受体表达的影响

目的:观察电磁脉冲(EMP)辐照亲代大鼠后其雄性子代下丘脑GABA<,A>受体表达的变化.方法:应用免疫组织化学及图像分析观察亲代大鼠接受 EMP 辐照后其雄性子代下丘脑 GABA<,A>受体表达的变化.结果:与对照组相比,100次脉冲组其雄性子代下丘脑室旁核GABA<,A>受体阳性神经元增加,400 次脉冲组光密度值显著增强;100次和400次脉冲组弓状核GABA<,A>受体阳性神经元增加,光密度值增强.结论:电磁脉冲可以影响雄性子代下丘脑GABA<,A>受体的表达,这可能与电磁脉冲辐射产生的远期遗传毒性有关.

Effects of Electromagnetic Pulse on the Expression of GABAA Receptor in Hypothalamus of Male Offsprings of Rats

Objective: To investigate the relationship between the expression of GABAA receptor in hypothalamus of the offspring of male rats and electromagnetic pulse (EMP). Methods: Immunocytochemistry combined with computer imaging analysis was carried out to examine the expression of the GABAA receptor in hypothalamus of male offspring. Results: Compared with the rats of control group, the expression of GABAA receptor increased in male offspring of 100 pulses group, the value of IOD increased in 400 pulses group; The expression of GABAA receptor in the arcuate nucleus increased. Conclusions: Electromagnetic pulse could induce the expression of GABAA receptor in hypothalamus of male offspring, which may be related to the long-term genetic toxicity of EMP.