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| 乳腺癌组织CCNA1 启动子区甲基化与肿瘤转移的相关性 | |
| 引用本文： | 宋美华,唐金海,吴建中,张晓梅,曹海霞.乳腺癌组织CCNA1 启动子区甲基化与肿瘤转移的相关性[J].现代生物医学进展,2011,11(7):1321-1326. |
| 作者姓名： | 宋美华 唐金海 吴建中 张晓梅 曹海霞 |
| 作者单位： | 1. 南京医科大学附属第一临床医学院,江苏,南京,210029 2. 江苏省肿瘤医院普外科,江苏,南京,210009 3. 江苏省肿瘤医院科研科,江苏,南京,210009 |
| 摘 要： | 目的:探讨CCNA1基因甲基化在乳腺癌发生发展中的作用。方法:采用Qiagen-FFPE的步骤提取95例石蜡包埋乳腺癌组织的基因组DNA,应用限制性酶切-PCR对所提DNA进行CCNA1甲基化检测。结果:乳腺癌组织中CCNA1基因启动子甲基化的阳性率为90.9%(86/95)。结论:CCNA1基因甲基化参与乳腺癌的发生,发展,有淋巴结转移多见的特征,CCNA1基因甲基化与乳腺癌转移由关。 |
| 关 键 词： | 乳腺癌 CyclinA1基因 甲基化 预后 |
Correlation of Cyclin A1 Gene Hypermethylation in Promoter Region and Metastasis of Breast Carcinoma |
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| SONG Mei-hu,TAN Jin-hai,WU Jian-zhong,ZHANG Xiao-mei,CAO Hai-xia.Correlation of Cyclin A1 Gene Hypermethylation in Promoter Region and Metastasis of Breast Carcinoma[J].Progress in Modern Biomedicine,2011,11(7):1321-1326. | |
| Authors: | SONG Mei-hu TAN Jin-hai WU Jian-zhong ZHANG Xiao-mei CAO Hai-xia |
| Institution: | 1 Department of the first clinical medical college Affiliated Nanjing Medical University,Jiangsu Nanjing,China,210029;2 Department of Surgery,the Affiliated Jiangsu cancer hospital of Nanjing medical university,Jiansu Nanjing,China,210009;3 Department of scientific research,Jiangsu cancer hospital,Jiangsu Nanjing,China,210009) |
| Abstract: | Objective: To investigate the methylation pattern of CCNA1 in breast carcinoma and explore the role of these epigenetic changes in tumorgenensis. Methods: 95 specimens of breast cancer were used, and the DNA was extracted by Qiagen procedure. Methylation change in the promoter region of CCNA1 was determined by using restricted enzyme cut-PCR. Results: The positive rate of gene promoter hypermethylation of CCNA1 was 90.9% (86/95). Conclusions: CCNA1 hypermethylation was correlated with lymph node metastasis, which may play an important role in pathogenesis of breast cancer. |
| Keywords: | Breast cancer Cyclin A1 Methylation Prognosis |
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