钙离子浓度动态变化

目的:探讨应用激光共聚焦扫描显微镜(LSCM)技术检测缺氧状态的人肺微血管内皮细胞(HPMVEC)内钙离子(Ca2+)浓度动态变化的价值。方法:HPMVEC常规培养,按观察时间点不同分为5个缺氧培养组(1h hyp组、2h hyp组、4h hyp组、6h hyp组和8h hyp组)以及1个对照组(0h con组)共6个组,每组设8个复孔,应用LSCM技术测定缺氧后HPMVEC内Ca2+浓度水平及随时间推移的变化。结果:LSCM技术显示HPMVEC内Ca2+的荧光强度1h hyp组与0h con组比较、2h hyp组与1h hyp组比较、4h hyp组与2h hyp组比较、6h hyp组与4h hyp组比较、8h hyp组与6h hyp组比较有显著差异(P<0.05)。线性回归分析结果显示Ca2+荧光强度与缺氧时间成正相关(r=0.969,P<0.01)。结论:HPMVEC内Ca2+浓度随缺氧时间增长而增高;LSCM在动态检测缺氧状态下HPMVEC内的Ca2+浓度变化中具有明显优势。

Detection of Dynamic Changes of Intracellular Calcium in Hypoxia Human Pulmonary Microvascular Endothelial Cells by Laser Scanning Confocal Microscope

Objective:To investigate the dynamic changes of intracellular calcium concentration(i) by laser scanning confocal microscope(LSCM) in hypoxia human pulmonary microvascular endothelial cells(HPMVEC).Methods:HPMVEC conventional culture,divided into six groups:one control group(Group 0h con) and five hypoxia groups,respectively hypoxia for 1h,2h,4h,6h and 8h(1h hyp group,2h hyp group,4h hyp group,6h hyp group and 8h hyp group),each group has 8 wells.The changes of the intracellular calcium concentration(i) wer...