Effects of insulin on pyruvate dehydrogenase in circulating lymphocytes from normal and diabetic rats |
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| Authors: | M T Rinaudo M Curto R Bruno C Marino M Mostert |
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| Institution: | Dipartimento di Medicina ed Oncologia Sperimentale, Facoltà di Medicina e Chirurgia, Università di Torino, Italy. |
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| Abstract: | 1. The in vivo and in vitro conditions which allow a response of rat circulating lymphocyte PDH to insulin are investigated. 2. In vivo tests show that inactive PDH (PDHi) prevails in diabetic rats and active PDH (PDHa) in hyperinsulinemic rats; in treated with insulin diabetic rats the PDHa/PDHi ratio (1.7) is similar to that of normal rats (PDHa/PDHi ratio = 2). 3. In vitro tests show a responsiveness of PDH to insulin only when 50 microM Ca2+ -Mg2+ and intact lymphocytes are used in the incubation medium. Insulin concentrations and contact time are important variables. |
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