Expression,characterization of recombinant human soluble BAFF secreted from CHO cell |
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| Authors: | H T Wu X M Yan Y L Hu Z Y Diao J Wu S Q Zhang |
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| Institution: | (1) Jiangsu Province Key Laboratory for Molecular & Medical Biotechnology, Nanjing Normal University, Nanjing, 210046, P.R. China;(2) Basic Medical College of Nanjing University of Chinese Medicine, Nanjing, 210029, P.R. China;(3) School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China |
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| Abstract: | B cell-activating factor of the TNF family (BAFF) is critical for B cell maturation and survival. Here, we constructed a stable CHO cell line, in which the expression level of soluble form of BAFF (sBAFF) was raised from 0.13 μg/ml to 0.55 μg/ml. Purified recombinant sBAFF from these CHO cells not only bound to its receptors but also co-stimulated the proliferation of human peripheral blood B lymphocyte in vitro. These results provided us with a useful basis for further studies about sBAFF-related research. |
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| Keywords: | B cell-activating factor of the TNF family B lymphocyte Chinese Hamster Ovary cell |
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