四季豆种子中两种蛋白质的分离纯化及其性质初步研究

四季豆（ＰｈａｓｅｏｌｕｓｖｕｌｇａｒｉｓＬ．）属于豆科蝶形花亚科野百合属，在我国分布极广，普植于温带地区．虽然四季豆是一种极普通的营养作物，但贮藏过久或煮沸不透常发生中毒．四季豆种子中成分复杂，除了蛋白组分外，还含有多种小分子组分［１］．一般认为，四季豆种子的毒性是这些组分复合作用的结果一直引人关注的则是其中的有毒蛋白．四季豆蛋白中很多是凝集素［２～４］，四季豆凝集素是一种典型的植物血细胞凝集素（Ｐｈｙｔｏｈｅｍａｇｇｌｕｔｉｎｉｎ，ＰＨＡ），是一种寡糖结合专一性的糖蛋白，具有凝聚血细胞，刺激…

Purification and Characterization of New Proteins from the Seeds of Phaseolus valgaris L

From the seeds of red kidney bean( Phaseolus valgaris L.),two novel protein constituents named PV3 and PV5 have been isolated and purified by extraction with PB buffer,S Sepharose Fast Flow column chromatography.These proteins are proved to be homogeneous by SDS PAGE、IEF and HPLC analysis,and presented the typical absorption spectra in ultraviolet region.The molecular weights of the two proteins were approximately 65 kD and 61 kD by HPLC analysis,but 34 kD and 31 kD by using SDS PAGE analysis,respectively.Later two values were in agreement with the amino acid compositions computation.The fact that there were no Cys in these proteins indicated that the subunit was the base composition unit.The isoelectric points of the two proteins were 6.0 and 6.5.The hemagglutinating activity of two proteins was founded.Protein PV3 and PV5 were crystallized using hanging drop vapor diffusion method.