A new set of lacZ fusion vectors, pUCPlac, for studying gene expression in Drosophila by P-mediated transformation |
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| Authors: | G Molsberger U Sch?fer M Sch?fer |
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| Affiliation: | 1. Zucker Hillside Hospital, Glen Oaks, NY, and Hofstra Northwell School of Medicine, Hempstead, NY;2. Otsuka Pharmaceutical Development and Commercialization, Inc., Princeton, NJ;1. Section of Epigenomics and Disease, Department of Medicine, Imperial College London, London W12 0NN, United Kingdom;2. Genomic Programming of Beta Cells Laboratory, Institut d’Investigacions Biomediques August Pi I Sunyer (IDIBAPS), Barcelona 08036, Spain;3. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Madrid 28029, Spain;4. Department of Genetics and Genomic Science, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA;5. Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut du cerveau et de la moelle (ICM) – Hôpital Pitié-Salpêtrière, Boulevard de l’Hôpital, Paris 75013, France;6. Germans Trias i Pujol University Hospital and Research Institute and Josep Carreras Leukaemia Research Institute, Badalona 08916, Spain;7. Diabetes, Obesity, and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA;8. European Genomic Institute for Diabetes, INSERM UMR 1190, Lille 59800, France;9. Diabetes Research Institute (HSR-DRI), San Raffaele Scientific Institute, Milano 20132, Italy;10. Cell Isolation and Transplantation Center, University of Geneva, 1211 Geneva 4, Switzerland;11. Department of Clinical Sciences, Lund University Diabetes Centre, Lund University, Lund 20502, Sweden;1. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA;2. Howard Hughes Medical Institute, 77 Avenue Louis Pasteur, Boston, MA 02115, USA;3. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;4. Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA;5. Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA;1. Institute of Cell Biology, IZB, University of Bern, Bern 3012, Switzerland;2. Polarity Division and Morphogenesis, Institut Curie, CNRS UMR 3215, INSERM U934 Paris, France |
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| Abstract: | A new vector, pUCPlac, was generated by introducing a truncated lacZ structural gene into pUChsneo [Steller and Pirrotta, EMBO J. 4 (1985) 167-171]. In front of this gene a new polylinker was added which will allow the in-frame fusion of sequences containing cis-acting regulatory elements plus translation start site of any given gene. After P-mediated germ-line transformation in Drosophila the action of these regulatory elements can be conveniently monitored by histochemical staining for beta-galactosidase activity. Correct cloning can be easily ascertained by supercoil plasmid sequencing of the constructs using commercially available primers. |
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