Characterization of ferritin and ferritin-binding proteins in canine serum |
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Authors: | Kiyotaka Watanabe Kazuhiro Hayashi Toshihiro Miyamoto Mina Tanaka Shozo Okano Shinji Yamamoto |
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Affiliation: | Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Japan. watanabe@vmas.kitasato-u.ac.jp |
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Abstract: | Ferritin and ferritin-binding proteins in canine serum were characterized. A certain percentage of ferritin in canine serum, but no tissue ferritin, was precipitated by centrifugation at 16,000×g for 30 min. The precipitated ferritin was found to contain two subunits corresponding to the H and L subunits of canine liver ferritin by immunoblotting, the H subunit being predominant. More ferritin was precipitated from canine sera which had been incubated with anti-rat liver ferritin antibody than from untreated sera, and the H chain also predominated. To evaluate the possibility that the autoantibody was responsible for the precipitation of canine serum ferritin, the ferritin-binding activities of canine antibodies were examined using liver ferritin-coated microtiter plates and alkaline phosphatase-labeled antibodies specific for canine IgM, IgA, and IgG heavy chains. The results showed that IgM and IgA, but not IgG, had considerable ferritin-binding activities. Given these results, we suggest that there is H-chain-rich isoferritin in canine serum, and that ferritin exists as an immune complex. |
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Keywords: | autoantibody canine serum ferritin-binding protein immune complex serum ferritin |
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