Enrichment of anammox bacteria from marine environment for the construction of a bioremediation reactor |
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Authors: | Jun Nakajima Makiko Sakka Tetsuya Kimura Kenji Furukawa Kazuo Sakka |
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Institution: | (1) Graduate School of Bioresources, Mie University, Tsu 514-8507, Japan;(2) Department of Civil Engineering and Architecture, Kumamoto University, Kumamoto 860-8555, Japan |
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Abstract: | In the global ocean nitrogen cycle, the anaerobic ammonium-oxidizing (anammox) process is recognized as important. In this
study, we established an enrichment culture of marine anammox bacteria (MAB) in a column-type reactor. The reactor, which
included a porous polyester non-woven fabric that had been placed at the sea floor in advance for enrichment, was continuously
fed with NH4Cl and NaNO2 for more than 1 year. Anammox activity in the MAB reactor was confirmed by 15N tracer analysis using 15NH4Cl and Na14NO2. We identified two 16S rRNA genes in the amplified DNA fragments derived from MAB, which were highly homologous with those
from Candidatus “Scalindua wagneri” and an uncultured planctomycete clone. Fluorescence in situ hybridization analysis using an anammox-specific
probe also confirmed that MAB predominated in the reactor. To our knowledge, this is the first report on the establishment
of an enrichment culture of anammox bacteria from the marine environment using a continuous culture system. |
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Keywords: | Anammox Denitrification Marine Candidatus “ Scalindua wagneri” FISH |
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