A rapid sensitive silver stain for polypeptides in polyacrylamide gels |
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| Authors: | C R Merril M L Dunau D Goldman |
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| Institution: | 1. Laboratory of General and Comparative Biochemistry, National Institute of Mental Health, Bethesda, Maryland 20205 USA;2. National Institute of Alcohol and Alcoholism, Bethesda, Maryland 20205 USA |
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| Abstract: | The use of silver to detect polypeptides was originally achieved by modifying tissue stains. By adapting methods of photochemistry we have developed a new silver stain for polypeptides which is nearly as sensitive but much more efficient than these earlier procedures. The new silver stain utilizes only three solutions and allows protein patterns to be visualized within 50 min. Its sensitivity is 100 times that of the Coomassie blue stain. |
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| Keywords: | To whom all correspondence should be addressed |
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