Synthesis and DNA binding properties of terminally modified peptide nucleic acids |
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| Authors: | Mokhir Andriy Zohm Burkhard Fuessl Andreas Kraemer Roland |
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| Affiliation: | Anorganisch-Chemisches Institut, Karl-Ruprechts University of Heidelberg, Im Neuenheimer Feld 270, Heidelberg 69120, Germany. |
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| Abstract: | PNAs with terminal modifications of varying structure and charge were synthesized and their binding to DNA was studied. A variation in thermal stability of 19. 8 degrees C has been observed between the least and the most stable PNA-DNA duplexes. The most stable duplex melts 7.7 degrees C higher than the duplex of the corresponding non-modified PNA and complementary DNA. It has been shown that sequence fidelity of the PNA conjugate having the highest DNA affinity is significantly better than that of non-modified PNA. The results obtained can be used for the design of PNA probes, whose binding to DNA is sequence independent. |
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