Two-dimensional electrophoretic profiling of atopic dermatitis in primary cultured keratinocytes from patients |
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Authors: | Park Yong-Doo Jang Hee-Sun Kim So-Yeon Ko Sung-Kyun Lyou You-Jeong Lee Dong-Youn Paik Young-Ki Yang Jun-Mo |
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Institution: | Department of Dermatology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Korea. |
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Abstract: | Recently, we reported altered protein expression in primary cultured fibroblasts from atopic dermatitis (AD) patients. As a sequential study, we conducted proteomic analysis of primary keratinocytes derived from AD patients to further identify AD-related proteins. Three pH ranges, 4-7, 6-9, and 7-11, were used to profile the altered protein expression in AD. We obtained 46 candidate spots from the 2-D gel image analysis: 18 proteins were up-regulated and 27 proteins were down-regulated. Among the several important candidate proteins, NCC27 showed the same profile of a defect in PTM in both AD-derived keratinocytes and fibroblasts. On the basis of current and previous reports, real-time PCR was performed on select candidate genes to compare RNA and protein expression levels in AD-derived keratinocytes and fibroblasts. Our results provide new clues to aid in understanding the mechanism of atopic alterations in keratinocytes and suggest new AD-associated proteins that are important in AD pathogenesis. |
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