The influence of vasoactive intestinal polypeptide and cholecystokinin of prolactin release in rat and human monolayer cultures |
| |
Authors: | William B Malarkey Thomas M ODorisio Michelle Kennedy Samuel Cataland |
| |
Institution: | Department of Medicine, Ohio State University Hospital 410 West Tenth Avenue, Columbus, Ohio 43210, USA |
| |
Abstract: | We have evaluated the effects of the gut-brain peptides, VIP and CCK, on pituitary PRL secretion in monolayer cultures of normal and tumor bearing rodent and human pituitary tissue. In cultures prepared with normal human pituitary tissue obtained from three patients with metastatic breast cancer, VIP at 10?7M and 10?9M (but not 10?11M) significantly (p<.05) increased PRL secretion in the wells by 6 hrs. Similar concentrations of VIP also significantly (p<.05) promoted PRL release from pituitary tissue obtained by transphenoidal hypophysectomy from one of two prolactinoma patients. Dopamine (10?5M) inhibition of PRL secretion was not affected by 10?11 to 10?7M VIP. In contrast to these findings VIP did not significantly influence 6 hr rat PRL release in monolayer cultures of normal or transformed cells (GH3) with or without the addition of bacitracin (10?5M).CCK33 significantly (p<.01) increased rat PRL release in human pituitary monolayer cultures at 10?5M. The more biologically potent CCK8 significantly (p<.02) increased rat PRL release at a 10-fold lower concentration, 10?6M. In contrast, CCK8 10?8 to 10?6M, did not significantly influence PRL release from normal human pituitary cultures or from tumor bearing human (prolactinoma) and rat (GH3) cultures. We conclude that 1) the gut-brain peptides, VIP and CCK, can directly stimulate pituitary PRL release and 2) VIP may be a physiologic prolactin releasing factor in man. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|