Effect of papain treatment on dipeptide transport into rabbit intestinal brush border vesicles |
| |
Authors: | Vadivel Ganapathy Joseph F. Mendicino Frederick H. Leibach |
| |
Affiliation: | Department of Cell and Molecular Biology Medical College of Georgia Augusta, Georgia 30912, USA |
| |
Abstract: | Purified rabbit intestinal brush border membrane vesicles transport glycyl-L-proline into an osmotically responsive intravesicular space by a Na+- independent, carrier-mediated process. With short incubation, transport occurs mostly as the intact dipeptide, followed by hydrolysis. Pretreatment of the vesicles with papain results in a 60% reduction of L-alanine transport while glycyl-L-proline transport is stimulated by 40%. Papain treatment does not change the intravesicular volume, nor does it increase membrane permeability. Dipeptide transport into papain treated vesicles remains completely Na+- independent as it is in the control vesicles. Many dipeptides inhibit glycyl-L-proline transport into papain treated vesicles both in the presence and absence of a Na+ gradient. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|