Direct binding of 3H-lisuride to adrenergic and serotonergic receptors |
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Authors: | George Battaglia Milt Titeler |
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Affiliation: | Department of Pharmacology University of Toronto Toronto, Ontario M5S 1A8, Canada |
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Abstract: | The characteristics of the specific binding of 3H-lisuride hydrogen maleate (3H-LHM) to homogenates of rat striatum and bovine frontal cortex tissue were investigated. In rat striatum 50% of 3H-LHM binding was inhibited potently by spiperone and haloperidol indicating a component of 3H-LHM binding to D2 dopamine receptors. Specific 3H-LHM binding was detected in rat striatum after selective blockade of the D2 dopamine component indicating specific 3H-LHM binding to other striatal sites. In bovine frontal cortex clonidine and serotonin competition curves for specific 3H-LHM binding included high affinity phases indicating alpha2 adrenergic and high affinity serotonergic components of binding. Blockade of the adrenergic component by 10?7M clonidine resulted in the specific 3H-LHM binding exhibiting distinctly serotonergic characteristics. Conversely, blockade of the serotonergic component by 2 × 10?7M serotonin resulted in the specific 3H-LHM binding exhibiting distinct alpha2 receptor characteristics. These data demonstrate the specific binding of 3H-LHM to alpha2 adrenergic receptors, to a high affinity serotonin site, and to D2 dopamine receptors. |
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