Construction of Streptococcus mutans glucosyltransferase mutants utilizing a cloned gene fragment |
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| Authors: | Setsuko Sato Shunsaku Ueda Howard K. Kuramitsu |
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| Affiliation: | Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, IL, U.S.A. |
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| Abstract: | Abstract An internal 1.6-kb BAM HI DNA fragment of the previously cloned Streptococcus mutans GS-5 gtfB gene was utilized to construct plasmids capable of insertion in toto into the GS-5 chromosome. The resultant insertions primarily yielded mutants defective in glucosyltransferase-I activity. These mutants were also defective in sucrose-dependent colonization of smooth surfaces. |
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| Keywords: | Streptococcus mutans Gene cloning Glucosyltransferase |
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