| 小麦与羊草体细胞杂种的细胞核和细胞质基因组分析 |
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| 引用本文： | 陈秀玲,夏光敏,陈惠民. 小麦与羊草体细胞杂种的细胞核和细胞质基因组分析[J]. 植物生理与分子生物学学报, 2004, 30(4): 379-386 |
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| 作者姓名： | 陈秀玲 夏光敏 陈惠民 |
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| 作者单位： | 山东大学生命科学学院,济南,250100 |
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| 摘 要： | 通过原生质体融合与培养获得小麦与羊草的体细胞杂种,其核基因组成以羊草(供体)为主,用线粒体特异探针atp6与叶绿体特异探针rbcL进行的RFLP分析结果表明,胞质基因组成偏向羊草并发生了重组.讨论了受体核基因组消减对杂种再生及对受体胞质基因组消减的影响.
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| 关 键 词： | 小麦 羊草 不对称体细胞杂交 核/质基因组 |
| 修稿时间： | 2003-07-29 |
Nuclear and Cytoplasmic Genome Analysis of Somatic Hybrid of Triticum aestivum L.and Leymus chinensis (Trin.) Tzvel. |
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| CHEN Xiu-Ling,XIA Guang-Min,CHEN Hui-Min. Nuclear and Cytoplasmic Genome Analysis of Somatic Hybrid of Triticum aestivum L.and Leymus chinensis (Trin.) Tzvel.[J]. Journal Of Plant Physiology and Molecular Biology, 2004, 30(4): 379-386 |
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| Authors: | CHEN Xiu-Ling XIA Guang-Min CHEN Hui-Min |
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| Affiliation: | School of Life Sciences, Shandong University, Jinan 250100, China. |
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| Abstract: | Intergeneric somatic hybrids were obtained by fusion between protoplasts of Triticum aestivum L. cv. Jinan 177 and Leymus chinensis (Trin.) Tzvel. Protoplasts of L. chinensis were exposed to UV (300 microW/cm(2)) for 30 s, 45 s and 1 min before fusion. The results of morphological and chromosomal observation, isozyme pattern as well as RAPD analysis and the 5S rDNA space sequence analysis showed the hybrid nature of the regenerated colonies of fusion combination T (+) L (UV 30 s). Restriction Fragment Length Po1ymorphism (RFLP) analysis using mitochondrion-specific atp6 and chloroplast-specific rbcL probes showed that most of the organelle genomes of the hybrids originated from L. chinensis and some recombination happened between wheat and L. chinensis. The effects of elimination of the nuclear genome of receptor protoplast on hybrid regeneration and receptor organelle genome elimination are discussed. |
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| Keywords: | common wheat Leymus chinensis asymmetric somatic hybridization nuclear and cytoplasmic genomes |
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