Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus |
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Authors: | Ishida Masami Oshima Tairo Yutani Katsuhide |
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Affiliation: | Biotechnology Research Centre, La Trobe University, 3552, Bendigo, Vic., Australia. |
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Abstract: | Micromanipulation was used to obtain an isolate (BEN 52) of Eikelboom Type 1851 from a bulking activated sludge plant. Its 16S rDNA sequence reveals its closest relative is 'Roseiflexus castenholzii', a member of the phylum 'Chloroflexi', class 'Chloroflexi', previously called the green non-sulfur bacteria. The 16S rRNA targeted oligonucleotide probe designed for fluorescence in situ hybridisation against this sequence successfully identified filamentous bacteria with the morphological features of Type 1851 in activated sludge samples from plants in several countries and different operational configurations. |
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Keywords: | Filamentous bacterium Activated sludge Bulking Type 1851 'Chloroflexi' Green non-sulfur bacterium Fluorescence in situ hybridization |
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