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Anomalous electrophoretic migration of oligodeoxynucleotides with terminal OH groups: Applications for DNA exonuclease characterization |
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| Authors: | Sofia P Becerra Sevilla D Detera Samuel H Wilson |
| Institution: | Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205 USA |
| Abstract: | Oligodeoxynucleotides with a terminal OH group on both the 5′ and 3′ ends migrate anomalously in 23% polyacrylamide-7 m urea gels. This migration anomaly can be exploited to characterize nuclease digestion products. Thus, using specific substrates and the methods described, several types of DNA exonuclease activity can be readily distinguished. |
| Keywords: | polyacrylamide-urea gel electrophoresis oligo(dA) oligo(dT) anomalous electrophoretic migration 3′ → 5′ DNA exonuclease 5′ → 3′ DNA exonuclease |
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