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Ca regulates the subcellular localization of adenomatous polyposis coli tumor suppressor protein
Authors:Tatsuru Togo
Institution:Department of Anatomy, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki, Kanagawa 216-8511, Japan
Abstract:Microtubule (MT) plus-end tracking proteins (+TIPs) are involved in the regulation of MT plus-end dynamics and stabilization. It was reported previously that an increase in intracellular Ca2+ concentration (Ca2+]i) induced by disruption of the plasma membrane stimulates rearrangement of MTs T. Togo, Disruption of the plasma membrane stimulates rearrangement of microtubules and lipid traffic toward the wound site, J. Cell Sci. 119 (2006) 2780-2786], suggesting that some +TIPs are regulated by Ca2+. In the present study, the behavior of adenomatous polyposis coli (APC) following an increase in Ca2+]i was observed using Xenopus A6 epithelial cell expressing GFP-tagged APC. An increase in Ca2+]i by cell membrane disruption or by ionomycin treatment induced dissociation of APC without depolymerizing MTs. Inhibition of a tyrosine kinase and GSK-3β suppressed APC dissociation upon an increase in Ca2+]i. Western blotting analysis showed that Ca2+ transients activated GSK-3β through a tyrosine kinase. These results suggest that Ca2+ stimulates redistribution of APC through a tyrosine kinase- and GSK-3β-dependent pathway.
Keywords:Microtubule  Adenomatous polyposis coli  Calcium  Tyrosine kinase  GSK-3β
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