Long-term high glucose concentration influences Akt, ERK1/2, and PTP1B protein expression in human aortic smooth muscle cells |
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Authors: | Doina Popov Miruna Nemecz Adriana Georgescu |
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Institution: | a Institute of Cellular Biology and Pathology “N. Simionescu”, 8, B.P. Hasdeu Street, Bucharest 050568, Romania b Institute of Molecular Cell Biology, Center for Molecular Biomedicine, Friederich Schiller University, Jena 07743, Germany |
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Abstract: | Hyperglycemia stimulates a plethora of intracellular signaling pathways within the cells of the vascular wall resulting in dysfunction-associated pathologies. Most of the studies reported so far explored the effect of rather short-time exposure of smooth muscle cells to high glucose concentrations. To mimic situation in Type 2 diabetes in which vascular wall is constantly exposed to circulating hyperglycemia, we report here the long-term (7 days) effect of high glucose concentration on human media artery smooth muscle cells. This consists in up-regulation of PTP1B protein expression, down-regulation of basal Akt phosphorylation, and elevation of basal ERK1/2 activation. Acute stimulation of cells in high glucose with insulin down-regulated PTP1B expression, slightly decreased ERK1/2 activity, and activated Akt, whereas oxidative stress up-regulated Akt and ERK1/2 phosphorylation. In conclusion, long-term high glucose and acute oxidative stress and insulin stimulation imbalance the expression of activated kinases Akt and ERK1/2 and of dephosphorylating PTP1B in the insulin signaling pathway. |
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Keywords: | Aortic media smooth muscle cells Hyperglycemia Insulin signaling Kinases Protein tyrosine phosphatase 1B |
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