|
|||||
|
|
Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish |
|
| Authors: | Chiou-Yueh Lee Shao-Yang Hu Mark Hung-Chih Chen Jen-Leih Wu |
| Institution: | a Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan, ROC b Department of Food Science, Taipei College of Maritime Technology, Taipei, Taiwan, ROC c Laboratory of Marine Molecular Biology and Biotechnology, Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, ROC d Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Keelung, Taiwan, ROC e Department of Biotechnology, Hungkuang University, Taichung, Taiwan, ROC |
| Abstract: | Myostatin belongs to the transforming growth factor (TGF)-β superfamily and is a potent negative regulator of skeletal muscle development and growth. We utilized microinjection of an antisense RNA-expressing vector to establish a hereditarily stable myostatin gene knockdown zebrafish strain with a double-muscle phenotype. Real-time PCR and immunostaining revealed that the myostatin messenger (m)RNA and protein levels in homozygous transgenic zebrafish were 33% and 26% those of the non-transgenic controls, respectively. Also, the mRNA levels of myogenic regulatory factor markers such as MyoD, myogenin, Mrf4, and Myf5 were dramatically elevated in myostatin-suppressed transgenic fish compared to the non-transgenic controls. Although there was no significant difference in body length, homozygous transgenic zebrafish were 45% heavier than non-transgenic controls. Histochemical analysis showed that the cross-sectional area of the muscle fiber of homozygous transgenic fish was twice as large as that of non-transgenic controls. This is the first model zebrafish with a hereditarily stable myostatin-suppressed genotype and a double-muscle phenotype. |
| Keywords: | Myostatin Zebrafish RNA interference |
| 本文献已被 ScienceDirect 等数据库收录！ | |