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内生真菌发酵提取物和植物生长调节剂对大豆根际细菌多样性的影响
引用本文:李冰宇,肖军,王红,李旭,马晓颖,贾东贝,杨涛.内生真菌发酵提取物和植物生长调节剂对大豆根际细菌多样性的影响[J].微生物学通报,2011,38(3):362-369.
作者姓名:李冰宇  肖军  王红  李旭  马晓颖  贾东贝  杨涛
作者单位:1. 沈阳农业大学土地与环境学院,辽宁沈阳,110161
2. 辽宁省农业科学院创新中心,辽宁沈阳,110161
3. 中科院沈阳应用生态研究所,分子生物学实验室,辽宁沈阳,110016
基金项目:辽宁省科技攻关项目(No. 009208001)
摘    要:为了研究内生真菌发酵提取物和植物生长调节剂对大豆根际细菌多样性的影响,采用PCR-DGGE技术分析了各处理中不同发育期的大豆根际细菌群落变化。结果发现发酵提取液和植物生长调节剂能增加部分优势菌群的数量,但对根际细菌类群结构影响并不明显;生育周期也是影响根际细菌数量的重要因素。另外割胶测序发现优势菌群主要是Proteobacteria(变形菌门)、Acidobacteria(酸杆菌纲)、Nitrospira(硝化螺旋菌属)、Bradyrhizobium(慢生根瘤菌属)等,这些也都是大豆根际比较常见的细菌类群。

关 键 词:PCR-DGGE  内生真菌  植物生长调节剂  根际细菌
收稿时间:2010/10/27 0:00:00
修稿时间:2010/12/10 0:00:00

Effects of endophytically fungal extracts and plant growth regulators on soybean rhizosphere bacterial diversity
LI Bing-Yu,XIAO Jun,WANG Hong,LI Xu,MA Xiao-Ying,JIA Dong-Bei and YANG Tao.Effects of endophytically fungal extracts and plant growth regulators on soybean rhizosphere bacterial diversity[J].Microbiology,2011,38(3):362-369.
Authors:LI Bing-Yu  XIAO Jun  WANG Hong  LI Xu  MA Xiao-Ying  JIA Dong-Bei and YANG Tao
Institution:College of Land and Environment, Shenyang Agriculture University, Shenyang, Liaoning 110161, China;Liaoning Academy of Agricultural Science, Shenyang, Liaoning 110161, China;Liaoning Academy of Agricultural Science, Shenyang, Liaoning 110161, China;Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China;College of Land and Environment, Shenyang Agriculture University, Shenyang, Liaoning 110161, China;College of Land and Environment, Shenyang Agriculture University, Shenyang, Liaoning 110161, China;Liaoning Academy of Agricultural Science, Shenyang, Liaoning 110161, China
Abstract:In order to study the effects of endophytically fungal extracts and plant growth regulators on soybean rhizosphere bacterial diversity, the dynamics of rhizosphere bacterial community in different developmental stages was studied based on PCR-DGGE approach. The results showed that they could increase some bacteria in populations; but the variation of rhizosphere bacterial diversity by fermentation extracts and plant growth regulator was no obvious, and the growth stages as an important factor that affects the rhizosphere bacteria. In addition, we found that the advantage bacteria mainly is Proteobacteria, Acidobacteria, Nitrospira, Bradyrhizobium, etc. These are the common bacterial species in the root of soybean.
Keywords:PCR-DGGE  Endophytically fungal extracrs  Plant growth regulators  Rhizosphere bacteria
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